Raptinal

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Raptinal 直接激活 caspase-3，可启动 caspase 依赖性的细胞凋亡内源性途径。Raptinal 能够通过直接激活效应 caspase-3来快速诱导癌细胞死亡，绕过启动子 caspase-8 和 caspase-9的激活。

Raptinal, a agent that directly activates caspase-3, initiates intrinsic pathway caspase-dependent apoptosis. Raptinal is able to rapidly induce cancer cell death by directly activating the effector caspase-3, bypassing the activation of initiator caspase-8 and caspase-9.

Cell Viability Assay Cell Line:Human Lymphoma U-937, SKW 6.4, or Jurkat cell lines Concentration:0.7-3.4 μM Incubation Time:24 hours Result:The IC50?values of Raptinal against U-937, SKW 6.4, or Jurkat cell lines were 1.1±0.1, 0.7±0.3, 2.7±0.9 μM, respectively.Western Blot Analysis Cell Line:Human gastric cancer cell lines AGS, MKN28, MKN45 Concentration:10?μM Incubation Time:2 hours Result:Induced apoptosis by activating caspase-3 within 30?min at a concentration of 10?μM. Treatment with 10?μM of Raptinal for 2?h induced the cleavage of pro-caspase-3 into it’s active form in all three cell lines.?

Animal Model:C57BL/6 and BALB/c female mice (6-8 weeks old) bearing the B16-F10 model or 4T1 modelsDosage:20 mg/kg Administration:Administered intraperitoneally; once daily for 3 consecutive days for B16-F10 and 4 consecutive days for 4T1 models Result:Retard tumor volume and tumor mass by 60% relative to controls in the B16-F10 model.Similar efficacy was observed for the 4T1 murine breast cancer tumor model with 50% growth inhibition after treatment.?

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Apoptosis

Caspase

Caspase

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1176-09-6

386.44

C28H18O2

>98% (HPLC)

In Vitro:?DMSO 中的溶解度 : 20 mg/mL (51.75 mM; 超声助溶 (<60°C)

O=CC1(c2ccccc2-c2ccccc12)C1(C=O)c2ccccc2-c2ccccc12

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(-20℃)

With Ice Pack

≥ 2 years