Ned 19

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Ned 19 是一种选择性膜透性非竞争性 NAADP 拮抗剂，抑制 NAADP 介导的 Ca2+ 信号传导，IC50 为 65 nM。 Ned 19 强烈抑制小鼠的肿瘤生长和血管形成以及肺转移。

Ned 19 is a selective membrane-permeant non competitive NAADP antagonist and inhibits NAADP-mediated Ca2+ signaling, with anIC50 of 65 nM. Ned 19 strongly inhibits tumor growth and vascularization as well as lung metastases in mice.

Ned 19 (25-100 μM; 24-72?hours) reduces cell proliferation.Ned 19 (25-100 μM; 24-72?hours) reduces markedly the cell number.Ned 19 (25-100 μM; 24-72?hours) reduces the S phase percentage and increases of the G0/G1 phase percentage evaluated by cell cycle analysis. Ned 19 (25-100 μM; 24-72?hours) induces cell apoptosis a time-dependent manner. Ned 19 (25-100 μM; 24-72?hours) reduces expression of N-cadherin and increases expression of E-cadherin, affecting the cell migratory behavior. Cell Proliferation Assay Cell Line:B16 cells Concentration:25, 50, 100 μM Incubation Time:24, 48, 72?hours Result:Reduced cell proliferation.Cell Viability Assay Cell Line:B16 cells Concentration:25, 50, 100 μM Incubation Time:24, 48, 72?hours Result:Reduced markedly the cell number. Cell Cycle Analysis Cell Line:B16 cells Concentration:25, 50, 100 μM Incubation Time:24, 48, 72?hours Result:Reduced the S phase percentage and increased of the G0/G1 phase percentage.Apoptosis AnalysisCell Line:B16 cells Concentration:25, 50, 100 μM Incubation Time:24, 48, 72?hours Result:Induced cell apoptosis a time-dependent manner.Western Blot Analysis Cell Line:B16 cells Concentration:25, 50, 100 μM Incubation Time:24, 48, 72?hours Result:Reduced expression of N-cadherin and increased expression of E-cadherin.

Ned 19 (i.p.; 5?mg/Kg/every second day; 4 week) impaires severely tumor growth. Animal Model:Adult male C57BL/6 mice Dosage:5?mg/Kg Administration:I.p.; every second day; 4 week Result:Impaired severely tumor growth.

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GPCR/G Protein

Calcium Channel

Calcium Channel

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874374-25-1

514.59

C30H31FN4O3

>98% (HPLC)

In Vitro:?DMSO 中的溶解度 : 125 mg/mL (242.91 mM; 超声助溶 )

C(O)(=O)C1CC2=C(C(N1)C3=CC(CN4CCN(CC4)C5=C(F)C=CC=C5)=C(OC)C=C3)NC=6C2=CC=CC6

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(-20℃)

With Ice Pack

≥ 2 years