HNHA

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

HNHA 是 HDAC 的抑制剂。

HNHA is an inhibitor of HDAC.(In Vitro):Treatment of cells with HNHA could elicite histone hyperacetylation resulting in an up-regulation of cell cycle arrest, p21 transcription, and an inhibition of HT1080 cell invasion. HNHA was able to inhibit in-vitro HDAC enzyme activity as well as proliferation of human fibrosarcoma cells (HT1080). (In Vivo):Several HDAC inhibitors, including MS-27-275 and a hydroxyamide-based HDAC inhibitors such as SAHA have been investigated extensively and shown to inhibit tumor growth in vivo.

HNHA (0-100 μM, 96 h) shows strong inhibition at lower concentrations on cancer cell lines, especially on breast cancer cells, mouse FM3A and human MCF-7.HNHA (15 μM, 24 h) arrests cancer cells at the G1/S phase of the cell cycle, activates p21and rescues strongly protein acetylation.HNHA (15 μM, 12 h) inhibits angiogenic proteins in breast cancer cells, effectively inactivates MMP-2, MMP-9, VEGF and HIF-1α. Cell Proliferation Assay Cell Line:FM3A, C1300, LA-N-1, LA-N-2, LA-N-5, NB16, NB19, NB69, SK-N-SH, MCF-7 and HT-29 Concentration:0-100 μM Incubation Time:96 h Result:Showed strong inhibition at lower concentrations on all cancer cell lines (FM3A, C1300, LA-N-1, LA-N-2, LA-N-5, NB16, NB19, NB69, SK-N-SH, MCF-7 and HT-29), with IC50 values of 15.70, 55.63, 22.78, 23.18, 26.70, 19.64, 21.26, 22.31, 65.09, 14.33, and 16.98 μM, respectively.Cell Viability Assay Cell Line:FM3A and MCF-7Concentration:0, 0.1, 1, 5, 10, 15, 20, 25,30 μM Incubation Time:48 h Result:Showed dose-dependent inhibition of viability in mouse and human breast cancer cells.Cell Cycle AnalysisCell Line:FM3A and MCF-7 cellsConcentration:15 μMIncubation Time:24 h Result:Arrested FM3A and MCF-7 cells in the G1/S phase.Western Blot Analysis Cell Line:FM3A and MCF-7 cells Concentration:0, 0.1, 1, 10, and 20 μM (24 h)Incubation Time:1, 6, 24, 48, and 72 h (15 μM) Result:Activated a cell proliferation arrestor p21, increased histone and non-histone protein acetylation and inhibited FM3A and MCF-7 proliferation in vitro, and was very effective in increasing the acetylation level of histone H3 protein in FM3A and MCF-7. The most effective dose point for acetylation of histone H3 was 10-20 μM. Histone H3 acetylation peaked after 1 h of exposure to the drugs and remained stable for 1-6 h.Western Blot Analysis Cell Line:FM3A and MCF-7 cells Concentration:15 μM Incubation Time:12 h Result:Showed a strong induction of TIMP-1 and TIMP-2, and effectively inactivated MMP-2, MMP-9, VEGF and HIF-1α.

HNHA (20 μM/mouse, IP, once every 2 days for a total of six injections) reduces tumor burden and extends the survival rate, activates TIMP-1, TIMP-2 and p21 and inhibits MMP-2, MMP-9, HIF-1α and VEGF protein expression. Animal Model:C3H/HeJ-FasL mice (FM3A breast cancer cell tumor xenograft, 6 weeks, n = 25/group)Dosage:20 μM/mouse Administration: IP, once every 2 days for a total of six injections Result:Reduced tumor burden and extended the survival rate. Effectively inhibited cancer development and angiogenesis in vivo. Increased TIMP-1, TIMP-2 and p21, decreased MMP-2, MMP-9, HIF-1α and VEGF protein expression, and reduced the distribution of CD34, HIF-1α and VEGF.

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Cell Cycle/DNA Damage

HDAC

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926908-04-5

303.42

C17H21NO2S

>98% (HPLC)

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ONC(=O)CCCCCCSc1ccc2ccccc2c1

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(-20℃)

With Ice Pack

≥ 2 years