NS19504

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

NS19504 是 Ca2+ 激活的 K+ 通道的激活剂，EC50 为 11.0 μM。 NS19504 对膀胱平滑肌自发阶段性收缩具有松弛作用。

NS19504 is an activator of Ca2+-activated K+ channel with EC50 of 11.0 μM. NS19504 shows relaxing effect on bladder smooth muscle spontaneous phasic contractions.(In Vitro):NS19504, which activated the BK channel with an EC50 value of 11.0 μM. Hit validation was performed using high-throughput electrophysiology (QPatch), and further characterization was achieved in manual whole-cell and inside-out patch-clamp studies in human embryonic kidney 293 cells expressing hBK channels: NS19504 caused distinct activation from a concentration of 0.3 and 10 M NS19504 left-shifted the voltage activation curve by 60 mV.?Furthermore, whole-cell recording showed that NS19504 activated BK channels in native smooth muscle cells from guinea pig urinary bladder.?In guinea pig urinary bladder strips, NS19504 (1 M) reduced spontaneous phasic contractions, an effect that was significantly inhibited by the specific BK channel blocker iberiotoxin.?In contrast, NS19504 (1 M) only modestly inhibited nerve-evoked contractions and had no effect on contractions induced by a high K(+) concentration consistent with a K(+) channel-mediated action.

NS19504 increases BK currents in freshly isolated guinea pig bladder smooth muscle cells and whole-cell. NS19504 increases the whole-cell currents response to 127 %, 194 %, 258 %, 561 % of control at a concentration of 0.32 μM, 1.0 μM, 3.2 μM and 10 μM, respectively.

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Cell Cycle/DNA Damage

Potassium Channel

Parasite

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327062-46-4

269.16

C10H9BrN2S

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (371.53 mM)

Nc1ncc(Cc2ccc(Br)cc2)s1

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(-20℃)

With Ice Pack

≥ 2 years