PBIT

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

PBIT 抑制 JARID1B 组蛋白去甲基酶（IC50 约为 3 μM）。 PBIT 还抑制 JARID1A 和 JARID1C（IC50 分别为 6 μM 和 4.9 μM）。 PBIT 是富含 Jumonji AT 的交互域 1 (JARID1) 酶的特异性抑制剂。 PBIT，在体外抑制 JARID1B，IC50 约为 3 μm。

PBIT inhibits JARID1B histone demethylase (IC50 of about 3 μM). PBIT also inhibits JARID1A and JARID1C (IC50s of 6 μM and 4.9 μM, respectively). PBIT is a specific inhibitor of the Jumonji AT-rich Interactive Domain 1 (JARID1) enzymes . PBIT, which inhibits JARID1B with an IC50 of about 3 μm in vitro.?Consistent with this, PBIT treatment inhibited removal of H3K4me3 by JARID1B in cells.?Furthermore, this compound inhibited proliferation of cells expressing higher levels of JARID1B.?

Cell Proliferation Assay Cell Line:Human breast cancer cell lines (UACC-812 and MCF7) and human mammary epithelial cells (MCF10A)Concentration:1, 3, and 10 μM for UACC-812 cells; 2.5, 5, and 10 μM for MCF7 and MCF10A cells Incubation Time:72 hours Result:Inhibited cell proliferation in a JARID1B level-dependent manner. 10 μM killed most of the UACC-812 cells, but showed minimal toxicity to MCF7 cells and MCF10A cells.

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Others

Other Targets

JARID1B|JARID1A|JARID1C

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2514-30-9

241.31

C14H11NOS

>98% (HPLC)

DMSO:100 mg/mL (414.40 mM; Need ultrasonic)

O=C1N(C2=CC=C(C)C=C2)SC3=C1C=CC=C3

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(-20℃)

With Ice Pack

≥ 2 years