LCH-7749944

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

LCH-7749944通过下调PAK4/c-Src/EGFR/cyclin D1通路有效抑制人胃癌细胞的增殖并诱导细胞凋亡。

LCH-7749944 effectively suppresses the proliferation of human gastric cancer cells through downregulation of PAK4/c-Src/EGFR/cyclin D1 pathway and induces apoptosis.?LCH-7749944 is a potent PAK4 inhibitor with an IC50 of 14.93 μM.?LCH-7749944 (5-20 μM; 12-48 hours) prominently induces a dose-dependent increase in the percentage of cells in G1 phase and decrease in S phase. ?LCH-7749944 (5-30 μM; 24 hours) dramatically decreases levels of phosphoPAK4, phospho-c-Src, phospho-EGFR and cyclin D1 protein expression in a dose-dependent manner.?LCH-7749944 (GNF-PF-2356;?5-50 μM;?24 hours) inhibits the proliferation of MKN-1, BGC823, SGC7901 and MGC803 cells in a concentration dependent manner.??LCH-7749944 (5-20 μM;?12-48 hours) induces apoptosis of SGC7901 cells.??

Cell Proliferation Assay Cell Line:MKN-1, BGC823, SGC7901 and MGC803 human gastric cancer cells Concentration:5, 10, 15, 20, 25, 30, 35, 40, 45, 50 μM Incubation Time:24 hoursResult:Inhibited the proliferation of MKN-1, BGC823, SGC7901 and MGC803 cells in a concentration dependent manner.Apoptosis Analysis Cell Line:SGC7901 cells Concentration:5, 10, 20 μM Incubation Time:12, 24, 48 hours Result:Induced apoptosis of SGC7901 cells.Cell Cycle Analysis Cell Line:SGC7901 cells Concentration:5, 10, 20 μM Incubation Time:12, 24, 48 hours Result:Prominently induced a dose-dependent increase in the percentage of cells in G1 phase and decrease in S phase.Western Blot AnalysisCell Line:SGC7901 cells Concentration:5, 10, 20, 30 μM Incubation Time:24 hours Result:Dramatically decreased levels of phosphoPAK4, phospho-c-Src, phospho-EGFR and cyclin D1 protein expression in a dose-dependent manner.

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GNF-PF-2356

Apoptosis

Apoptosis

PAK4|apoptosis

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796888-12-5

350.41

C20H22N4O2

>98% (HPLC)

DMSO:250 mg/mL (713.45 mM; Need ultrasonic)

COc1cccc(Nc2nc(NCC3CCCO3)c3ccccc3n2)c1

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(-20℃)

With Ice Pack

≥ 2 years