Daphnetin

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Daphnetin 是一种天然香豆素衍生物，是一种蛋白激酶抑制剂，抑制 EGFR、PKA 和 PKC，IC50 分别为 7.67 μM、9.33 μM 和 25.01 μM，还具有抗炎和抗氧化活性。

Daphnetin, a natural coumarin derivative, is a protein kinase inhibitor, inhibits EGFR, PKA and PKC with IC50 of 7.67 μM, 9.33 μM and 25.01 μM, respectively, also known to exhibit anti-inflammatory and anti-oxidant activities.(In Vitro):Daphnetin (7,8-dihydroxycoumarin) (0-40 μg/mL; 24-48 hours) inhibits the proliferation of ovarian cancer cells.Daphnetin (7,8-dihydroxycoumarin) (0-40 μg/mL; 24 hours; A2780 cells) induces apoptosis and increases ROS production in a dose-dependent manner.Daphnetin (7,8-dihydroxycoumarin) (0-40 μg/mL; 24 hours; A2780 cells) induces autophagy through modulation of the AMPK/Akt/mTOR pathway.Daphnetin (7,8-dihydroxycoumarin) (1-10 μM; plasmodium falciparum) exhibits schizontocidal activity in a dose-dependent manner. (In Vivo):Daphnetin (7,8-dihydroxycoumarin) (30 mg/kg; i.p.; daily; for 12 days; BALB/c nude mice) has antitumour activities in a xenograft animal model.Daphnetin (7,8-dihydroxycoumarin) (2.5-10 mg/kg; i.p.; daily; for three days; C57BL/6 mice) inhibits cisplatin-induced inflammation, decreases TNF-α, IL-1β, ROS and MDA production in a dose-dependent manner in kidney tissues. Daphnetin inhibits cisplatin-induced NF-κB activation and up-regulated Nrf2 and HO-1.Daphnetin (7,8-dihydroxycoumarin) (10-100 mg/kg; i.g. and i.p.; every four days, for 30 days; male Kunming outbred strain mice) displays certain schizontocidal activity in vivo.

Daphnetin (7,8-dihydroxycoumarin) (0-40 μg/mL; 24-48 hours) inhibits the proliferation of ovarian cancer cells.Daphnetin (7,8-dihydroxycoumarin) (0-40 μg/mL; 24 hours; A2780 cells) induces apoptosis and increases ROS production in a dose-dependent manner.Daphnetin (7,8-dihydroxycoumarin) (0-40 μg/mL; 24 hours; A2780 cells) induces autophagy through modulation of the AMPK/Akt/mTOR pathway.Daphnetin (7,8-dihydroxycoumarin) (1-10 μM; plasmodium falciparum) exhibits schizontocidal activity in a dose-dependent manner. Cell Viability Assay Cell Line:IOSE8C, A2780, SKOV3 and OVCAR8 cells Concentration:0, 5, 10, 20 and 40 μg/mL Incubation Time:24 h and 48 hours Result:Inhibited growth in ovarian cancer cells but not in normal cells.Apoptosis AnalysisCell Line:A2780 and SKOV3 cells Concentration:0, 10, 20 and 40 μg/mL Incubation Time: 24 hours Result:Increased apoptosis in a dose-dependent manner in A2780 and SKOV3 cells.Western Blot Analysis Cell Line:A2780 and SKOV3 cells Concentration:0, 10, 20 and 40 μg/mL Incubation Time:24 hours Result:Increased proapoptotic protein (Caspase 3, Bax, and PARP) expression but decreased antiapoptotic protein (Bcl2) expression.Western Blot Analysis Cell Line:A2780 cells Concentration:0, 10, 20 and 40 μg/mL Incubation Time:24 hours Result:Increased LC3 II and p62 expression in a dose-dependent manner and reduced the expression levels of p-Akt, p-mTOR, but increased the expression level of p-AMPK.

Daphnetin (7,8-dihydroxycoumarin) (30 mg/kg; i.p.; daily; for 12 days; BALB/c nude mice) has antitumour activities in a xenograft animal model.Daphnetin (7,8-dihydroxycoumarin) (2.5-10 mg/kg; i.p.; daily; for three days; C57BL/6 mice) inhibits cisplatin-induced inflammation, decreases TNF-α, IL-1β, ROS and MDA production in a dose-dependent manner in kidney tissues. Daphnetin inhibits cisplatin-induced NF-κB activation and up-regulated Nrf2 and HO-1.Daphnetin (7,8-dihydroxycoumarin) (10-100 mg/kg; i.g. and i.p.; every four days, for 30 days; male Kunming outbred strain mice) displays certain schizontocidal activity in vivo. Animal Model:BALB/c nude mice Dosage:30 mg/kg Administration:Intraperitoneal injection; Daily; for 12 days Result:Decreased tumor volume and weight in a xenograft animal model.Animal Model:Male Kunming outbred strain mice Dosage:10, 50 or 100 mg/kg Administration:Oral gavage and intraperitoneal injection; every four days, for 30 days Result:Reduced the number of parasites in mice.

7,8-Dihydroxycoumarin | NSC 633563

Angiogenesis

EGFR

EGFR| PKA| PKC

Inflammation/Immunology

——

486-35-1

178.14

C9H6O4

>98% (HPLC)

DMSO: 35 mg/mL (196.47 mM)

O=C1C=CC2=CC=C(O)C(O)=C2O1

7,8-dihydroxy-2H-1-benzopyran-2-one

(-20℃)

With Ice Pack

≥ 2 years