N-Ethylmaleimide

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

N-乙基马来酰亚胺是一种广泛用于实验生化研究的巯基试剂。

N-ethylmaleimide is a sulfhydryl reagent that is widely used in experimental biochemical studies.(In Vitro):N-Ethylmaleimide (20 μM;30 min) inhibits Akt Ser-473, Akt Thr-308 , p70S6K, ribosomal protein S6, 4E-BP1, eIF4E, BAD and FKHR-L1 phosphorylation.N-Ethylmaleimide (20 μM;30 min) affects conversion of pro-caspase-3 in vascular smooth muscle cells.N-Ethylmaleimide (20 μM;6 h) promotes vascular smooth muscle cells apoptosis.N-Ethylmaleimide (20 μM;30 min) affects PP2A activity and ROS production in vascular smooth muscle cells.(In Vivo):N-Ethylmaleimide (10 mg/kg; i.h.) promotes the prevalence situation of mice with acute gastric ulcers.

N-Ethylmaleimide (20 μM;30 min) inhibits Akt Ser-473, Akt Thr-308 , p70S6K, ribosomal protein S6, 4E-BP1, eIF4E, BAD and FKHR-L1 phosphorylation.N-Ethylmaleimide (20 μM;30 min) affects conversion of pro-caspase-3 in vascular smooth muscle cells.N-Ethylmaleimide (20 μM;6 h) promotes vascular smooth muscle cells apoptosis.N-Ethylmaleimide (20 μM;30 min) affects PP2A activity and ROS production in vascular smooth muscle cells.Western Blot Analysis Cell Line:Vascular smooth muscle cellsConcentration:20 μMIncubation Time:2 hours Result:Effectively inhibited platelet-derived growth factor-BB (PDGF-BB)-stimulated Akt Ser-473 , Akt Thr-308, p70S6K, ribosomal protein S6, 4E-BP1, BAD and FKHR-L1 phosphorylation with a concentration of 20 μM.Western Blot Analysis Cell Line:Vascular smooth muscle cells Concentration:20 μM Incubation Time:2 hours Result:Increased of 1.8-fold in the conversion of pro-caspase-3 into active form, and showed better effect with 20 ng/ml PDGF-BB adding.Apoptosis Analysis Cell Line:Vascular smooth muscle cells Concentration:20 μM Incubation Time:6 hours Result:Induced vascular smooth muscle cells apoptosis by 3-fold, and exhibited 5-fold apoptosis with 20 ng/ml PDGF-BB adding.Cell Viability Assay Cell Line:Vascular smooth muscle cells Concentration:20 μM Incubation Time:30 min Result:Increased PP2A activity 1.7-flod and increased ROS production 2-fold in vascular smooth muscle cells.

N-Ethylmaleimide (10 mg/kg; i.h.) promotes the prevalence situation of mice with acute gastric ulcers. Animal Model:Male Wistar rats with acute gastric ulcers induced by absolute ethanol injection Dosage:10 mg/kg Administration:Subcutaneous injection; 10 mg/kg once Result:Increased the lesion area of acute gastric ulcers and attenuated the gastroprotective effect of PAG in rats.

——

Proteasome/Ubiquitin

Cysteine Protease

Cysteine protease

——

——

128-53-0

125.13

C6H7NO2

>98% (HPLC)

DMSO: 10 mM

CCN1C(=O)C=CC1=O

——

(-20℃)

With Ice Pack

≥ 2 years