FM19G11

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

缺氧诱导因子 (HIF) α-亚基抑制剂（缺氧诱导荧光素酶测定中 IC50 = 80 nM）。抑制 HIFα 亚型的转录活性。

Hypoxia inducible factor (HIF) α-subunit inhibitor (IC50 = 80 nM in hypoxia induced luciferase assay). Inhibits transcriptional activity of HIFα isoforms. Directly regulates expression and inhibits transcriptional activity of pluripotency markers (Sox2 and Oct4) in rodent and human stem cells under hypoxic conditions, driving differentiation. Improves locomotion after severe spinal cord injury in mice, alone or in combination with transplantation of ependymal stem cells of the spinal cord. Also inhibits O6-methylguanine DNA methyltransferase (MGMT) via HIF1α pathway, under normal and hypoxic conditions in glioblastoma cell lines.(In Vitro):FM19G11 (500 nM) promotes oligodendrocyte differentiation under hypoxic conditions. FM19G11 (300 nM) significantly enhances the pro-apoptotic effect of temozolomide, whereas FM19G11 does not induce apoptosis. In hypoxic GBM-XD, hypoxic T98G, and normoxic T98G cells, FM19G11 (300 nM) significantly inhibited the mRNA level of O6-methylguanine DNA-methyltransferase via the HIF-1α pathway.(In Vivo):Intramedullary injection of FM19G11 improves locomotion in severe spinal cord injury and induces the expression of GAP43 and RIP at the injury.

FM19G11 (30-300 nM) inhibits HIFα proteins in the HeLa cell lines.FM19G11 (500 nM) promotes oligodendrocyte differentiation under hypoxia.FM19G11 (300 nM; 3 days) suppresses the mRNA levels of O6-methylguanine DNA-methyltransferase (MGMT) significantly in hypoxic GBM‐XD, hypoxic T98G, and normoxic T98G cells.M19G11 (300 nM; 3 days) significantly enhances the pro‐apoptotic effect of temozolomide (TMZ), although FM19G11 does not induce apoptosis by itself.Cell Viability Assay Cell Line:GBM‐XD and T98G cells Concentration:300 nM Incubation Time:3 days Result:Had no cytotoxicity by itself.Enhanced the cytotoxicity of TMZ in hypoxic GBM-XD cells, hypoxic T98G cells, and normoxic T98G cells.Western Blot Analysis Cell Line:GBM‐XD and T98G cells Concentration:300 nM Incubation Time:3 days Result:Suppressed MGMT expression significantly in both cell lines in hypoxic culture.

FM19G11 ( intramedullary injection; 1-8 weeks) improves locomotion in severe spinal cord injury (SCI).FM19G11 ( intramedullary injection; 8 weeks) induces the expression of GAP43, an axon growth marker, and RIP, a marker for myelinated oligodendrocytes at the injury.

HIF-1alpha/2alpha Inhibitor

Others

Other Targets

5-HT1B|5-HT1D

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329932-55-0

463.402

C23H17N3O8

>98% (HPLC)

In Vitro:?DMSO : 100 mg/mL (215.80 mM)

Cc1ccc(cc1)C(=O)COC(=O)c1cccc(NC(=O)c2ccc(cc2[N+]([O-])=O)[N+]([O-])=O)c1

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(-20℃)

With Ice Pack

≥ 2 years