BML-210

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

一种新型组蛋白脱乙酰酶 (HDAC) 抑制剂，可在 A549 细胞中诱导 Ac-组蛋白，EC50 为 181 uM。

A novel histone deacetylase (HDAC) inhibitor that induces Ac-histone in A549 cells with EC50 of 181 uM; inhibits the growth of all cell lines and promotes apoptosis in the human leukemia cell lines (NB4, HL-60, THP-1, and K562); induces growth inhibition and apoptosis and downregulates HDAC1-5 and DAPC complex expression levels in cervical cancer cells.

Cell Proliferation AssayCell Line:NB4 cells Concentration:10, 20 μM Incubation Time:24, 48 hours Result:Inhibited cell proliferation and growth inhibition of NB4 cells in a dose- and time-dependent manner.Cell Cycle AnalysisCell Line:NB4 cells Concentration:10, 20 μM Incubation Time:24, 48 hours Result:Caused a decrease in the proportion of NB4 cells in the S phase and an increase in the G0/G1 phase.Caused an increase in the G0/G1 phase up to 70% at 24 and 48 h with 10 μM.Cell Cytotoxicity Assay Cell Line:NB4 cells Concentration:10, 20 μM Incubation Time:24, 48 hours Result:Caused cytotoxic effects on NB4 cells in a dose- and time-dependent manner.Apoptosis Analysis Cell Line:NB4 cells Concentration:10, 20 μM Incubation Time:24, 48 hours Result:At a dose of 10 μM induced apoptotic cell death.Western Blot Analysis Cell Line:NB4 cells Concentration:10, 20 μM Incubation Time:24, 48 hours Result:At 10 μM dose inhibited HDAC1 gene expression up to 36% after 48 h of treatment Inhibited HDAC expression up to 74% at 8 h point at 20 μM.

Animal Model:Female C57BL/6 mice with mouse breast cancer EO771 cells Dosage:20 mg/kg Administration:IP; three times per week for two weeks Result:Notably suppressed the tumour growth and weight.

BML210 | BML 210

Cell Cycle/DNA Damage

HDAC

HDAC

Cancer

——

537034-17-6

339.4314

C20H25N3O2

>98% (HPLC)

DMSO: ≥ 30 mg/mL

O=C(NC1=CC=CC=C1N)CCCCCCC(NC2=CC=CC=C2)=O

Octanediamide, N1-(2-aminophenyl)-N8-phenyl-

(-20℃)

With Ice Pack

≥ 2 years